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Image Search Results
Journal: Frontiers in Oncology
Article Title: TFAP2C-Mediated lncRNA PCAT1 Inhibits Ferroptosis in Docetaxel-Resistant Prostate Cancer Through c-Myc/miR-25-3p/SLC7A11 Signaling
doi: 10.3389/fonc.2022.862015
Figure Lengend Snippet: TFAP2C transcriptionally activates PCAT1 expression in DTX-resistant cells. (A) Venn diagram showing the possible transcription factors within the promoter regions of PCAT1 by online bioinformatics analysis (HUMAN TFDB and ALGGEN). (B-D) The mRNA and protein levels of TFAP2C were detected by qRT-PCR and Western blot in DTX-resistant PCa cells and their sensitive cells. (E) The mRNA levels of TFAP2C, PCAT1 and SLC7A11 in PC3/DR cells were detected after transfected with siTFAP2C and control siRNA. (F) qRT-PCR assay was used to assess the expression of TFAP2C, PCAT1 and SLC7A11 in PC3 cells after TFAP2C overexpression. (G) Schematic diagram showing location of TFAP2C binding sites on the promoter regions of PCAT1. (H) ChIP-qPCR was used to detect the binding efficiency of TFAP2C on the PCAT1 promoter. (I) The luciferase activities were measured in TFAP2C overexpressed PC3 cells after co-transfected with PCAT1 wild-type and mutant reporter plasmids. (J) Cell viability was assessed by CCK-8 assay in TFAP2C-knockdown PC3 cells after transfected with PCAT1 and vector plasmids. (K) The relative expression of TFAP2C was measured by qRT-PCR in 30 pairs of clinical PCa samples. (L) The positive relation between TFAP2C and PCAT1 in clinical samples (n=30) by qRT-PCR. (M) The schematic diagram showing that TFAP2C-induced PCAT1 inhibits ferroptosis in DTX-resistant PCa cells through c-Myc/miR-25-3p/SLC7A11 signaling. The data are presented as the mean ± SD, * P < 0.05.
Article Snippet: The primary antibodies were as follows: SLC7A11 (1:1000, Abcam, USA, Cat. ab175186), GPX4 (1:1000, Abcam, USA, Cat. ab125066),
Techniques: Expressing, Quantitative RT-PCR, Western Blot, Transfection, Control, Over Expression, Binding Assay, ChIP-qPCR, Luciferase, Mutagenesis, CCK-8 Assay, Knockdown, Plasmid Preparation